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> HEPES Buffer, Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)C8H18N2O4SMr 238.31CAS Number [7365-45-9]EC Number 230-907-9Beilstein Registry Number 883043Merck 14,4654RTECS TL6809000MDL Number MFCD00006158PubChem Substance ID 24895616Purity ≥ 99.5%pKa 7.5 at 25°C, Useful pH range of HEPES buffer 6.8 - 8.2, Titrate HR2-585 to the desired pH between 6.8 - 8.2 using NaOHHR2-585 Measured pH Range: 5.4 - 5.5 at 25°CHR2-585 Measured Conductivity Range: 160.1 - 236.9 μS/cm at 25°CHR2-585 Measured Refractive Index Range: 1.37093 - 1.37131 at 20°C, HR2-785 is titrated to pH 7.0 using NaOHHR2-785 Measured Conductivity Range: 5.8 - 7.0 mS/cm at 25°CHR2-785 Measured Refractive Index Range: 1.37133 - 1.37176 at 20°C, HR2-729 is titrated to pH 7.5 using NaOHHR2-729 Measured Conductivity Range: 11.6 - 13.6 mS/cm at 25°CHR2-729 Measured Refractive Index Range: 1.37177 - 1.37290 at 20°C, Insoluble Matter 0.01% maxDNase None DetectedRNase None DetectedProtease None DetectedHeavy Metals 1 ppm maxAs 5 ppm maxCu 5 ppm maxFe 5 ppm maxPb 5 ppm maxSO4 0.005% max. Proceedings of the National Academy of Sciences, 98(12), 6923-6928. To prepare L of HEPES Buffer ( M, pH 6.8~8.2 ) Input buffer volume, molar concentration to get formula. This chemical, HEPES (CAS 7365-45-9), is not tested for Pyrogens. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Popular Answers (1) 23rd Jan, 2014. HEPES has been described as one of the best all-purpose buffers available for biological research. We enable science by offering product choice, services, process excellence and our people make it happen. Your email subscription to News & Announcements has been received. Hortic., 36, 157–163. HEPES has been used in a wide variety of applications, including tissue culture. The VWR Traceable Logger-Trac Temperature Datalogger is perfect for monitoring material during storage, handling, and transportation. Quanta Biosciences and VWR are proud to fund a grant award for publication of research involving qPCR methodologies utilizing any Quanta Bioscience qPCR reagent in JoVE. 2015. We are sad to see you go. CopyCopied, Validated by Experts, Validated by Users, Non-Validated, Removed by Users, Predicted data is generated using the ACD/Labs Percepta Platform - PhysChem Module, Predicted data is generated using the US Environmental Protection Agency’s EPISuite™, Click to predict properties on the Chemicalize site, For medical information relating to Covid-19, please consult the,,, ACD/Labs Percepta Platform - PhysChem Module, US Environmental Protection Agency’s EPISuite™, Compounds with the same molecular formula, Search Google for structures with same skeleton. Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995. 2006. Available from 2.5 to 18L, two styles & three temperatures with a broad range of accessories. Cheers. Available at, 13 Koerner, M. M., Palacio, L. A., Wright, J. W., Schweitzer, K. S., Ray, B. D. and Petrache, H. I (2011) Biophys. Therefore, these chemicals are widely used in cell culture to maintain the pH of experiments under control. HEPES is also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid, and N-[2-Hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid]. (2007) Free Radical Biol. J. PMID: 2314256, Adobe Acrobat Reader is required to reliably view, print and comment on PDF documents. This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. A. and Bloksberg, L. N. (1988) Sci. PMID: 59429502. Dependable -80C storage with a focus on sustainability without sacrificing sample protection. Enzymol. To add items to your cart, enter a quantity and click Add to Cart. We collected academic references in which biological buffers were used for cell culture in order to help you understanding which buffers are more suitable for your applications. I've been buying this product from Santacruz for the last couple of years. 10: 155. Binders, calendars, pens, cleaning and sanitation supplies, and office equipment are just some of the essential products we offer... Science education supplies, specimens, activities, and equipment for all grade levels – kindergarten to college. Biochem. Multi purpose buffer used in biological research. PMID: # 30886219  Graf, S.|Brzezinski, P.|von Ballmoos, C.| et al. Biochem. 182: 50-68. Crystallization Plates, Hardware & Accessories, Crystallization grade HEPES buffer for formulating screens or for optimization, Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL). 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, C1CN(CCN1CCO)CCS(=O)(=O)O You can select and customize services for peak efficiency, quality, and accelerated innovation. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. (PubMed ID 11306489) utilized HEPES in their hypotonic lysis buffer used to lyse BMSC's. How is savings calculated?We What is the critical micelle concentration (CMC)? Enzymol. Learn more about HEPES, 1.0M buffer soln., pH 9.0. Subscribe to our mail list and be the first to receive exclusive content from Hopax in your mailbox: Be the first to know about Hopax's special offers, new blog posts and relevant news about our industry. Used under license. Macromolecules. PMID: 51042403. 5: 467-477. Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Useful pH range of HEPES buffer 6.8 - 8.2. 5 at 20 C. Electrophoresis, 22(6), 1004-1007. Uses of HEPES Buffer: This Good's buffer is a very popular buffering agent in cell culture medias . et al. PloS one. For In Vitro Diagnostic Use. HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. 8: 1219-26. Thermo Fisher Scientific, Don't have an account ? Available at, 6 Parfitt, D. E., Almehdi, A. Design by 37design, Taiwan Hopax Chemicals Mfg. Good, N.E., et al. > Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Journal of the National Cancer Institute. 2015. Used to buffer mammalian cell cultures in open systems of high density cultures and in closed systems of high and low density cultures, Used in cell culture media for experiments with dinoflagellates (e.g.

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